The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 15, 2022
Filed:
Jun. 11, 2018
Suzhou Maximum Bio-tech Co., Ltd, Suzhou, CN;
SUZHOU MAXIMUM BIO-TECH CO., LTD, Suzhou, CN;
Abstract
Gene knockout method based on base editing and its application is provided. The gene knockout method comprises: selecting a 20 bp-NGG target sequence of the coding region of the gene to be knocked out, so that it contains a complete target codon CAA, CAG or CGA; and using sgRNA sequence to locate BE3 to the target sequence, to convert the target single-base C of the target codon into T and thus introduce a corresponding termination codon TAA, TAG orTGA in order to realize the knockout, wherein the target single-base C is located preferably on site 4-8 in the target sequence, the interval between the target codon and NGG is 12 to 14 bp, and the upstream base (H) near the target codon cannot be G; and the sgRNA sequence is a 20 bp sequence complementary to the target sequence.