The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 15, 2022

Filed:

Apr. 20, 2017
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Jeremy W. Thorner, Berkeley, CA (US);

Gregory Charles Finnigan, San Francisco, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 15/90 (2006.01); C12N 15/09 (2006.01); C12N 15/79 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 15/09 (2013.01); C12N 15/102 (2013.01); C12N 15/11 (2013.01); C12N 15/79 (2013.01); C12N 15/81 (2013.01); C12N 15/90 (2013.01); C12N 2310/20 (2017.05);
Abstract

The present disclosure provides genetically modified cells and non-human organisms, and methods of producing such cells and organisms. Also provided are methods of editing the genome of such cells and organisms. The cells and non-human organisms of the disclosure are genetically modified such that their genome includes an integrated heterologous nucleic acid (that includes a CRISPR/Cas target sequence) at one or more (e.g., 2 or more, 3 or more, 4 or more, etc.) positions within the genome. The integrated nucleic acids, which include the same CRISPR/Cas target sequence, allow for simultaneous gene editing at multiple different positions within a genome using a single species of CRISPR/Cas guide RNA (i.e., one guide RNA will target multiple sites because the multiple sites have the same target sequence).


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