The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 08, 2022
Filed:
Apr. 09, 2019
Shandong Landsee Genetics Co., Ltd., Rizhao, CN;
Kui Li, Beijing, CN;
Yulian Mu, Beijing, CN;
Zhiguo Liu, Beijing, CN;
Julang Li, Guelph, CA;
Kui Xu, Beijing, CN;
Yinghui Wei, Beijing, CN;
Changli Ge, Rizhao, CN;
Xuehui Cai, Harbin, CN;
Long Wang, Rizhao, CN;
Qimei Chen, Rizhao, CN;
Yang Qiu, Rizhao, CN;
Shandong Landsee Genetics Co., Ltd., Rizhao, CN;
Abstract
Provided is a double-gene knockout vector system, a method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out, prepared porcine fibroblasts, and a method for preparing a gene-edited pig with both CD163 gene and CD13 gene being knocked-out. The vector system of the present disclosure comprises a CD163 gene knockout vector and a CD13 gene knockout vector. The CD163 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, with a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 1-3. The CD13 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 4-6.