The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 18, 2022

Filed:

Jul. 10, 2019
Applicant:

Imcyse SA, Liège, BE;

Inventors:

Jean-Marie Saint-Remy, Grez-Doiceau, BE;

Vincent Carlier, Enines, BE;

Luc Vander Elst, Obaix, BE;

Assignee:

IMCYSE SA, Liege, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); C07K 14/73 (2006.01); C07K 14/725 (2006.01); C07K 17/14 (2006.01); G01N 33/50 (2006.01); C07K 14/74 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56972 (2013.01); C07K 14/7051 (2013.01); C07K 14/70514 (2013.01); C07K 14/70539 (2013.01); C07K 17/14 (2013.01); G01N 33/505 (2013.01); G01N 33/56977 (2013.01); A61K 2039/605 (2013.01); C07K 2319/00 (2013.01); G01N 2333/70514 (2013.01); G01N 2333/70539 (2013.01); G01N 2469/10 (2013.01);
Abstract

The present invention relates an in vitro method for detecting class II restricted CD4+ T cells in a sample. Herein a sample is contacted with an isolated complex of an MHC class II molecule and a peptide. This peptide comprises an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif. CD4+ T cells are detected by measuring the binding of the complex with cells in the sample, wherein the binding of the complex to a cell is indicative for the presence of CD4+ T cells in the sample. The present invention further relates to an isolated complex of an MHC Class II molecule and a peptide comprising an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif.


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