The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 18, 2022
Filed:
Nov. 07, 2019
Becton Dickinson and Company, Franklin Lakes, NJ (US);
Susan M. Kircher, Hanover, PA (US);
Vanda White, Baltimore, MD (US);
William B. Brasso, Columbia, MD (US);
Dyan Luper, Cockeysville, MD (US);
James Y. Zhou, Columbia, MD (US);
Julie L. Rosales, Randallstown, MD (US);
Jeffery H. Bruton, Randallstown, MD (US);
John D. Mantlo, Sykesville, MD (US);
Adrien P. Malick, Granite, MD (US);
Donald R. Callihan, Cockeysville, MD (US);
Ben Turng, Tucson, AZ (US);
Liping Feng, Baltimore, MD (US);
Curtis M. Gosnell, Fallston, MD (US);
Patrick Shawn Beaty, Dallastown, PA (US);
John P. Douglass, York, PA (US);
BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US);
Abstract
Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.