The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 04, 2022
Filed:
Apr. 07, 2020
Applicant:
Bolt Threads, Inc., Emeryville, CA (US);
Inventors:
Matthew Scott Gamboa, Richmond, CA (US);
Joshua Tyler Kittleson, Pleasant Hill, CA (US);
Assignee:
Bolt Threads, Inc., Emeryville, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/60 (2006.01); C07K 14/435 (2006.01);
U.S. Cl.
CPC ...
C12N 9/60 (2013.01); C07K 14/43518 (2013.01); C12Y 304/23041 (2013.01);
Abstract
Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.