The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 04, 2022

Filed:

May. 19, 2017
Applicant:

Molly Shoichet, Toronto, CA;

Inventors:

Molly Shoichet, Toronto, CA;

Malgosia M. Pakulska, Toronto, CA;

Irja Elliott Donaghue, Whitby, CA;

Jaclyn M. Obermeyer, Toronto, CA;

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 9/51 (2006.01); A61K 9/06 (2006.01); A61K 9/14 (2006.01); A61K 38/18 (2006.01); A61K 38/19 (2006.01); A61K 47/36 (2006.01); A61K 47/38 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
A61K 9/5153 (2013.01); A61K 9/06 (2013.01); A61K 9/146 (2013.01); A61K 38/185 (2013.01); A61K 38/1816 (2013.01); A61K 38/195 (2013.01); A61K 47/36 (2013.01); A61K 47/38 (2013.01); A61K 38/00 (2013.01);
Abstract

The present disclosure provides a delivery system for controlled protein release without encapsulation. Identical, burst-free, extended release profiles for three different protein therapeutics were obtained with and without encapsulation in PLGA nanoparticles embedded within a hydrogel. Using both positively and negatively charged proteins, it was shown that short-range electrostatic interactions between the proteins and the PLGA nanoparticles are the underlying mechanism for controlled release. Moreover, tunable release was demonstrated by modifying nanoparticle concentration, nanoparticle size, or environmental pH. Additionally, the utility of this system was demonstrated in vivo for BDNF delivery in a rat model of stroke. These new insights obviate the need for encapsulation and offer promising, translatable strategies for more effective delivery of therapeutic biomolecules.


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