The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 28, 2021

Filed:

Dec. 23, 2016
Applicant:

University of Florida Research Foundation, Incorporated, Gainesville, FL (US);

Inventors:

Nathalie Clement, Gainesville, FL (US);

Barry John Byrne, Gainesville, FL (US);

Laura A. Small, Gainesville, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C12N 15/86 (2013.01); C12N 2710/16644 (2013.01); C12N 2750/14151 (2013.01);
Abstract

Provided herein are methods of improving rAAV production in cells, the method comprising increasing the salt concentration in the media in which the cells are infected or transfected, cultured, or in which they produce AAV. Aspects of the disclosure relate to improved methods of rAAV production by co-infecting suspension adapted cells (e.g., suspension adapted HEK293 cells) with viruses that encode one or more AAV components for producing rAAV particles within the suspension adapted cells.


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