The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 28, 2021

Filed:

Nov. 30, 2016
Applicant:

Kikkoman Corporation, Chiba, JP;

Inventors:

Yosuke Masakari, Chiba, JP;

Seiichi Hara, Chiba, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/80 (2006.01); C12Q 1/32 (2006.01); C12N 15/09 (2006.01); C12Q 1/00 (2006.01); C12N 9/04 (2006.01); C07K 19/00 (2006.01); C12P 21/02 (2006.01); G01N 27/327 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C07K 14/80 (2013.01); C07K 19/00 (2013.01); C12N 9/0006 (2013.01); C12N 15/09 (2013.01); C12N 15/102 (2013.01); C12P 21/02 (2013.01); C12Q 1/006 (2013.01); C12Q 1/32 (2013.01); G01N 27/327 (2013.01); C07K 2319/00 (2013.01); C07K 2319/70 (2013.01); G01N 2333/904 (2013.01);
Abstract

A cytochrome b-glucose dehydrogenase fusion protein having modified electron transfer properties, and a glucose measurement method and measuring kit using the cytochrome b-glucose dehydrogenase fusion protein are provided. Provided are a cytochrome b-glucose dehydrogenase fusion protein in which glucose dehydrogenase having homology with SEQ ID NO: 1 or SEQ ID NO: 4 and cytochrome b are linked together, as well as a glucose measurement method, a measurement reagent kit and a sensor using the cytochrome b-glucose dehydrogenase fusion protein. The cytochrome b-glucose dehydrogenase fusion protein of the present invention has modified electron transfer properties, and can be used for measuring glucose in the presence of a free-form mediator in reduced concentration or in the absence of a free-form mediator, and can be used, for example, in continuous glucose monitoring.


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