The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 21, 2021
Filed:
Jun. 17, 2016
Applicant:
Riken, Saitama, JP;
Inventors:
Atsushi Miyawaki, Saitama, JP;
Hiroyuki Katayama, Saitama, JP;
Assignee:
RIKEN, Saitama, JP;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/533 (2006.01); G01N 33/542 (2006.01); G01N 33/84 (2006.01); G01N 21/64 (2006.01); C07K 14/435 (2006.01); C07K 19/00 (2006.01); C12Q 1/06 (2006.01); C07K 14/00 (2006.01); C12Q 1/00 (2006.01); C12N 15/09 (2006.01); C12N 15/88 (2006.01); C12Q 1/02 (2006.01); C12Q 1/37 (2006.01);
U.S. Cl.
CPC ...
C07K 14/43504 (2013.01); C07K 14/00 (2013.01); C07K 19/00 (2013.01); C12N 15/09 (2013.01); C12N 15/88 (2013.01); C12Q 1/00 (2013.01); C12Q 1/025 (2013.01); C12Q 1/06 (2013.01); C12Q 1/37 (2013.01); G01N 33/533 (2013.01); G01N 33/542 (2013.01); G01N 33/84 (2013.01); G01N 21/6408 (2013.01); G01N 21/6428 (2013.01);
Abstract
The present invention provides a tool which exhibits excellent properties in the quantification of autophagy activity. A unimolecular FRET probe of the present invention includes an acceptor consisting of a fluorescent protein to be enzymatically degraded inside a lysosome or a vacuole; and a donor having an amino acid sequence having a sequence identity of 95% or more with respect to an amino acid sequence represented by SEQ ID NO: 1.