The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 21, 2021
Filed:
Nov. 30, 2016
Illumina, Inc., San Diego, CA (US);
Arash Jamshidi, San Francisco, CA (US);
Yan-you Lin, San Francisco, CA (US);
Farnaz Absalan, San Francisco, CA (US);
Sarah Stuart, San Francisco, CA (US);
Gordon Cann, San Francisco, CA (US);
Yir-Shyuan Wu, San Francisco, CA (US);
Tarun Khurana, San Francisco, CA (US);
Jeffrey S Fisher, San Francisco, CA (US);
Illumina, Inc., San Diego, CA (US);
Abstract
In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.