The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2021
Filed:
May. 20, 2020
Infinitus (China) Company Ltd., Guangdong, CN;
Zehua Chen, Guangdong, CN;
Yong Zhou, Guangdong, CN;
Jian Tang, Guangdong, CN;
Yonghua Wang, Guangdong, CN;
Zheng Xiao, Guangdong, CN;
Chenfeng Jing, Guangdong, CN;
Dong Liang, Guangdong, CN;
INFINITUS (CHINA) COMPANY LTD., Guangdong, CN;
Abstract
Provided is a method of producing an oil containing polyunsaturated fatty acids by usingsp. The method comprises: performing fermentation usingsp.; resuspending the resultant cells after fermentation in water, adding cellulase and neutral protease for enzymolysis; mixing the enzymatic hydrolyzate with n-hexane, shaking, extracting, centrifuging, and collecting the n-hexane phase; concentrating the n-hexane phase under reduced pressure to remove the n-hexane, and drying to obtain an oil; performing a first crystallization and a second crystallization, and then performing cold filtration to obtain a liquid oil after the second crystallization; and subjecting the liquid oil to deacidification and decolorization. By regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in thesp. is increased to more than 12%, and the obtained oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid.