The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2021
Filed:
Oct. 25, 2018
Viet Nam National University Ho Chi Minh City, Ho Chi Minh, VN;
Phuong Thao Thi Dang, Ho Chi Minh, VN;
MY Trinh Thi Nguyen, Hue, VN;
Nghia Hieu Nguyen, Kien Giang, VN;
Thuoc Linh Tran, Ho Chi Minh, VN;
Phuong Thao Thi Dang, Ho Chi Minh, VN;
My Trinh Thi Nguyen, Hue, VN;
Nghia Hieu Nguyen, Kien Giang, VN;
Thuoc Linh Tran, Ho Chi Minh, VN;
VIET NAM NATIONAL UNIVERSITY HO CHI MINH CITY, Thu Duc, VN;
Abstract
The present invention provides novel reagents and a cloning procedure based on homologous recombination for the site-directed cloning of a DNA fragment to a vector at designed site(s). The cloning reagents are made of mixture of extracts from at least two different cell types, preferably a mixture made of extracts from wild-typeand. Due to the activity of the mixture of cell extracts, recombination occurs between the 3' and 5′-ends of the target DNA and at the ends of linearized vector, which facilitates in-frame construction of expression vectors.