The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 14, 2021

Filed:

Jan. 31, 2019
Applicant:

Dsm Ip Assets B.v., Heerlen, NL;

Inventors:
Assignee:

DSM IP ASSETS B.V., Heerlen, NL;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12N 1/18 (2006.01); C12N 1/22 (2006.01); C12N 9/04 (2006.01); C12N 9/12 (2006.01); C12N 9/90 (2006.01); C12N 9/92 (2006.01); C12P 1/02 (2006.01);
U.S. Cl.
CPC ...
C12N 1/18 (2013.01); C12N 1/22 (2013.01); C12N 9/0006 (2013.01); C12N 9/1205 (2013.01); C12N 9/90 (2013.01); C12N 9/92 (2013.01); C12N 15/52 (2013.01); C12P 1/02 (2013.01); C12Y 101/01 (2013.01); C12Y 101/01307 (2013.01); C12Y 207/01016 (2013.01); C12Y 207/01017 (2013.01); C12Y 501/03001 (2013.01); C12Y 501/03004 (2013.01); C12Y 503/01004 (2013.01); C12Y 503/01005 (2013.01); C12Y 503/01009 (2013.01);
Abstract

The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.


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