The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2021
Filed:
May. 11, 2018
Institute of Hydrobiology, Chinese Academy of Sciences, Hubei, CN;
Guitang Wang, Hubei, CN;
Weishan Zhao, Hubei, CN;
Ming Li, Hubei, CN;
Shangong Wu, Hubei, CN;
Hong Zou, Hubei, CN;
Wenxiang Li, Hubei, CN;
Fan Xiong, Hubei, CN;
Abstract
A medium for culturingin vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl ofsuspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collectedinto a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating thecontinuously.are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the