The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 07, 2021
Filed:
May. 09, 2019
Applicants:
Shaofeng Ding, Santa Fe Springs, CA (US);
Qiang Liu, Rancho Cucamonga, CA (US);
Inventors:
Shaofeng Ding, Santa Fe Springs, CA (US);
Tony Zhou Lee, San Diego, CA (US);
Qiang Liu, Rancho Cucamonga, CA (US);
Assignee:
Other;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6853 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6858 (2013.01);
Abstract
Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.