The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 07, 2021

Filed:

Jan. 29, 2016
Applicant:

The University of North Carolina AT Chapel Hill, Chapel Hill, NC (US);

Inventors:

Nancy Allbritton, Chapel Hill, NC (US);

Yuli Wang, Cary, NC (US);

Christopher Sims, Chapel Hill, NC (US);

Scott Magness, Chapel Hill, NC (US);

Scott Bultman, Chapel Hill, NC (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12Q 1/08 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0697 (2013.01); C12N 5/0679 (2013.01); C12Q 1/08 (2013.01); C12N 2501/11 (2013.01); C12N 2501/15 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2513/00 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01); C12N 2537/10 (2013.01); G01N 2500/10 (2013.01);
Abstract

A method of making a live cell construct is carried out by: (a) providing a non-cellular support having a top surface and a bottom surface, (b) contacting live undifferentiated cells to the non-cellular support, and then (c) propagating a gastrointestinal epithelial cell monolayer on said top surface. In some embodiments, the live cells in the monolayer include: (i) undifferentiated cells (e.g., stem or progenitor cells); and (ii) optionally, but in some embodiments preferably, differentiated cells (e.g., enterocytes, Paneth cells, enteroendocrine cells, tuft cells, microcells, intra-epithelial lymphocytes, and/or goblet cells). Constructs formed by such methods and methods of using the same (e.g., in high through-put screening) are also described.


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