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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11182893 B1

PDFFull Text
Date of Patent:
Nov. 23, 2021

Filed:

Nov. 27, 2017

Method of pretreating sample including biological particles, method of acquiring image of biological particles, apparatus for pretreating sample including biological particles, and biological particle image-acquiring apparatus

Applicant:

Japan Agency for Marine-earth Science and Technology, Yokosuka, JP;

Inventors:

Tomo Kitahashi, Yokosuka, JP;

Hiromi Watanabe, Yokosuka, JP;

Masashi Tsuchiya, Yokosuka, JP;

Hiroyuki Yamamoto, Yokosuka, JP;

Assignee:

Japan Agency for Marine-Earth Science and Technology, Yokosuka, JP;

Attorney:

Wolf, Greenfield & Sacks, P.C.

Primary Examiner:

Maureen Wallenhorst

Int. Cl.
CPC ...
G01N 15/00 (2006.01); G01N 15/14 (2006.01); G01N 1/10 (2006.01); G01N 21/64 (2006.01); G06T 7/00 (2017.01); C12M 1/00 (2006.01); C12N 1/02 (2006.01); B82Y 5/00 (2011.01);
U.S. Cl.
CPC ...
G06T 7/0012 (2013.01); C12M 1/00 (2013.01); C12N 1/02 (2013.01); G01N 1/10 (2013.01); G01N 15/00 (2013.01); G01N 21/6458 (2013.01); B82Y 5/00 (2013.01); G01N 15/1459 (2013.01); G01N 15/1463 (2013.01); Y10T 436/255 (2015.01); Y10T 436/25375 (2015.01);
Abstract

A method of pretreating a sample including biological particles, the method including a step of acquiring a fraction (b) which passes through a sieve (A) having meshes of 250 to 1000 μm and does not pass through a sieve (B) having meshes of 32 to 63 μm by sieving a sample including biological particles as a detection target, and a step of adding a colloidal solution having a density of 1.10 to 2.45 g/cmto the fraction (b), subjecting the resultant solution to centrifugation, and acquiring a supernatant fraction (S) after the centrifugation.

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