The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 23, 2021

Filed:

Oct. 18, 2019
Applicant:

Omniome, Inc., San Diego, CA (US);

Inventors:

Hari K. K. Subramanian, San Diego, CA (US);

Chad Fleischer, San Diego, CA (US);

Denis Malyshev, San Diego, CA (US);

Assignee:

OMNIOME, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/682 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6811 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6869 (2013.01); C12Q 2521/101 (2013.01); C12Q 2525/203 (2013.01); C12Q 2525/30 (2013.01); C12Q 2527/113 (2013.01); C12Q 2527/137 (2013.01); C12Q 2533/101 (2013.01); C12Q 2600/16 (2013.01);
Abstract

A method for identifying a nucleic acid template that includes (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3' end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.


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