The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 23, 2021

Filed:

Feb. 27, 2018
Applicant:

Duke University, Durham, NC (US);

Inventors:

Michael David Lynch, Durham, NC (US);

Romel Menacho Melgar, Durham, NC (US);

Assignee:

Duke University, Durham, NC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C07K 7/06 (2006.01); C07K 14/50 (2006.01); C07K 14/505 (2006.01); C07K 14/535 (2006.01); C07K 14/555 (2006.01); C07K 14/575 (2006.01); C07K 14/59 (2006.01); C07K 14/605 (2006.01); C07K 14/61 (2006.01); C07K 14/62 (2006.01); C07K 14/755 (2006.01); C12N 9/16 (2006.01); C12N 9/64 (2006.01); C12N 9/82 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1029 (2013.01); C07K 7/06 (2013.01); C07K 14/50 (2013.01); C07K 14/505 (2013.01); C07K 14/535 (2013.01); C07K 14/555 (2013.01); C07K 14/575 (2013.01); C07K 14/57527 (2013.01); C07K 14/57545 (2013.01); C07K 14/59 (2013.01); C07K 14/605 (2013.01); C07K 14/61 (2013.01); C07K 14/62 (2013.01); C07K 14/755 (2013.01); C12N 9/16 (2013.01); C12N 9/644 (2013.01); C12N 9/82 (2013.01); C12P 21/00 (2013.01); C12Y 203/01016 (2013.01); C12Y 203/01097 (2013.01); C12Y 301/0202 (2013.01); C12Y 304/21022 (2013.01); C12Y 305/01001 (2013.01); C07K 2319/20 (2013.01); C07K 2319/50 (2013.01);
Abstract

Described are a genetically modified microorganism and corresponding methods and products. The genetically modified microorganism may include a first gene that encodes an acyl transferase and a second gene that encodes a peptide or protein. One or both of the first and second gene may be heterologous. The genetically modified microorganism may include a modified acyl-CoA biosynthetic pathway configured for one or more of: inducible biosynthesis of an acyl-CoA and over-accumulation of the acyl-CoA. The genetically modified microorganism may be effective upon fermentation to cause acylation of the peptide or protein by the acyl transferase using the acyl-CoA to provide a N-acylated peptide or protein product.


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