The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 23, 2021

Filed:

Sep. 23, 2016
Applicant:

University of the Witwatersrand, Johhannesburg, Johannesburg, ZA;

Inventors:

Kulsum Kondiah, Johannesburg, ZA;

Paul John Franklyn, Springs, ZA;

Vidya Keshav, Johannesburg, ZA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C02F 1/28 (2006.01); C02F 1/42 (2006.01); C07K 14/195 (2006.01); C02F 101/20 (2006.01); C02F 103/10 (2006.01); C02F 3/34 (2006.01);
U.S. Cl.
CPC ...
C02F 1/286 (2013.01); C02F 1/42 (2013.01); C02F 3/34 (2013.01); C07K 14/195 (2013.01); C02F 2001/425 (2013.01); C02F 2101/20 (2013.01); C02F 2103/10 (2013.01); C02F 2201/006 (2013.01); C02F 2305/08 (2013.01);
Abstract

A process for removing lead from a liquid is provided. The liquid is brought into contact with PbrD proteins having an amino acid sequence which has at least 80% identity to SEQ ID NO: 2, and these proteins bind to lead ions present in the liquid, thus removing the lead ions from the liquid. The bound lead ions are subsequently recovered, such as in the form of an insoluble salt or compound or by cation exchange chromatography, and can then be recycled. The PbrD protein can be immobilized before being brought into contact with the liquid. The proteins are typically immobilized in a matrix of nanoparticles. The PbrD proteins can be recombinantly expressed, such as in a bacterial system (e.g.) or a yeast expression system.


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