The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 16, 2021

Filed:

Mar. 15, 2018
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Omar Abudayyeh, Cambridge, MA (US);

James Joseph Collins, Newton, MA (US);

Jonathan Gootenberg, Cambridge, MA (US);

Feng Zhang, Cambridge, MA (US);

Eric S. Lander, Cambridge, MA (US);

Aviv Regev, Cambridge, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6883 (2018.01); C12Q 1/6886 (2018.01); C12N 15/11 (2006.01); C12Q 1/6888 (2018.01); C12Q 1/689 (2018.01); C12N 9/22 (2006.01); C12Q 1/6827 (2018.01); C12Q 1/6804 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6883 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6886 (2013.01); C12Q 1/6888 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.


Find Patent Forward Citations

Loading…