The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 16, 2021
Filed:
Jun. 27, 2019
Applicant:
Howard Hughes Medical Institute, Chevy Chase, MD (US);
Inventors:
Robert Singer, New York, NY (US);
Timothee Lionnet, Sterling, VA (US);
Wulan Deng, Ashburn, VA (US);
Assignee:
HOWARD HUGHES MEDICAL INSTITUTE, Chevy Chase, MD (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/683 (2018.01); C12Q 1/6841 (2018.01); C12Q 1/6804 (2018.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12P 19/34 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6855 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/683 (2013.01); C07H 21/04 (2013.01); C12N 15/09 (2013.01); C12P 19/34 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6841 (2013.01); C12Q 1/6855 (2013.01); C12Q 2531/113 (2013.01);
Abstract
Labeled probes, and methods of use thereof, comprise a Cas polypeptide conjugated to gRNA that is specific for target nucleic acid sequences, including genomic DNA sequences. The probes and methods can be used to label nucleic acid sequences without global DNA denaturation.