The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 16, 2021
Filed:
May. 20, 2016
University of Maryland, College Park, MD (US);
Canon U.s.a., Inc., Melville, NY (US);
Ian M. White, Ellicott City, MD (US);
Srinivasa Raghavan, Columbia, MD (US);
Kunal R. Pandit, Laurel, MD (US);
Imaly Nanayakkara, College Park, MD (US);
Weidong Cao, North Potomac, MD (US);
University of Maryland, College Park, College Park, MD (US);
Canon U.S.A., Inc., Melville, NY (US);
Abstract
The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.