The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 16, 2021
Filed:
Sep. 29, 2020
Mammoth Biosciences, Inc., South San Francisco, CA (US);
Lucas Benjamin Harrington, Berkeley, CA (US);
Janice Sha Chen, San Francisco, CA (US);
James Paul Broughton, San Francisco, CA (US);
Pedro Patrick Draper Galarza, Oakland, CA (US);
Wiputra J. Hartono, San Francisco, CA (US);
Isaac Paterson Witte, Walnut Creek, CA (US);
Jasmeet Singh, Santa Clara, CA (US);
MAMMOTH BIOSCIENCES, INC., South San Francisco, CA (US);
Abstract
Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.