The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 09, 2021
Filed:
Sep. 05, 2018
Department of Biotechnology, New Delhi, IN;
Council of Scientific and Industrial Research, New Delhi, IN;
Lakshmi Prasanna Gangavaramu, Telangana, IN;
Madhusudana Rao Nalam, Telangana, IN;
Veerabhadrav Rao Arravapalli, Telangana, IN;
DEPARTMENT OF BIOTECHNOLOGY, New Delhi, IN;
COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH, New Delhi, IN;
Abstract
The present invention discloses a recombinant, modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO.4 or SEQ ID NO.5. The recombinant, modified chitinase is derived by inserting two-point mutations at positions 661 and 2158 in the native chitinase gene ofLAK 1210 which results in amino acid substitution of tyrosine (Y) residue with histidine (H) at positions 221 and 720. The modified chitinase exhibits both exochitinase and endochitinase activity. The recombinant, modified enzyme is thermoactive with a temperature optimum of 55-60° C. and high thermostability (Tm of 66.7° C.), functions in a broad pH range (pH 3.0-11.0) having a pH optimum of 9.0 and also exhibits improved solubility and enhanced efficacy for control of insects and phytopathogenic fungi. The invention further provides improved methods for large scale production of recombinant modified chitinase and rapid, cost-effective purification method by chitin-adsorption affinity chromatography using powdered crustacean shells.