The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 02, 2021
Filed:
May. 12, 2017
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Yinhua Zhang, North Reading, MA (US);
Nathan Tanner, Peabody, MA (US);
Thomas C. Evans, Jr., Topsfield, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6876 (2018.01); G01N 33/52 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6846 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6876 (2013.01); G01N 33/52 (2013.01); C12Q 2527/119 (2013.01); C12Q 2527/125 (2013.01); C12Q 2545/114 (2013.01); C12Q 2563/173 (2013.01); C12Y 207/07007 (2013.01);
Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and Tris buffer in the range of 1.5 mM Tris to 5 mM Tris or equivalent.