The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Dec. 22, 2017
Applicant:

Xcella Biosciences, Inc., Menlo Park, CA (US);

Inventor:

Bob Chen, East Palo Alto, CA (US);

Assignee:

xCella Biosciences, Inc., Emeryville, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 35/10 (2006.01); B01L 9/00 (2006.01); G01N 15/14 (2006.01); G01N 33/543 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/6851 (2018.01); C40B 60/02 (2006.01); G01N 21/75 (2006.01); G01N 35/00 (2006.01); G01N 21/64 (2006.01); G01N 21/03 (2006.01); G01N 15/10 (2006.01); G01N 21/77 (2006.01);
U.S. Cl.
CPC ...
G01N 35/10 (2013.01); B01J 19/0046 (2013.01); B01L 3/50857 (2013.01); B01L 3/502715 (2013.01); B01L 9/523 (2013.01); C12N 15/1003 (2013.01); C12Q 1/6851 (2013.01); C40B 60/02 (2013.01); G01N 15/1475 (2013.01); G01N 15/1484 (2013.01); G01N 21/75 (2013.01); G01N 33/54386 (2013.01); B01J 2219/0097 (2013.01); B01J 2219/00306 (2013.01); B01J 2219/00509 (2013.01); B01J 2219/00511 (2013.01); B01J 2219/00524 (2013.01); B01J 2219/00587 (2013.01); B01J 2219/00599 (2013.01); B01J 2219/00684 (2013.01); B01J 2219/00722 (2013.01); B01J 2219/00869 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/142 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); C12Q 2531/113 (2013.01); G01N 15/1463 (2013.01); G01N 21/6428 (2013.01); G01N 2015/1006 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/755 (2013.01); G01N 2021/7769 (2013.01); G01N 2035/00158 (2013.01); G01N 2035/1039 (2013.01); G01N 2035/1053 (2013.01);
Abstract

Multi-stage sample-recovery systems, including automated 2-stage and 3-stage sample-recovery systems, are provided. Such systems enable the rapid screening and recovery of samples, including viable cell-based samples, from high-throughput screening systems, including systems utilizing large-scale arrays of microcapillaries. In specific screening systems, each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be identified and recovered using the multi-stage systems disclosed herein.


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