The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Jul. 06, 2016
Applicants:

Georgia State University Research Foundation, Inc., Atlanta, GA (US);

The United States Government As Represented BY the Department of Veterans Affairs, Washington, DC (US);

Inventors:

Gangli Wang, Brookhaven, GA (US);

Tanyu Wang, Atlanta, GA (US);

Didier Merlin, Decatur, GA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/416 (2006.01); C12Q 1/68 (2018.01); G01N 27/48 (2006.01); C12Q 1/6886 (2018.01); C12Q 1/682 (2018.01);
U.S. Cl.
CPC ...
G01N 27/416 (2013.01); C12Q 1/68 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6886 (2013.01); G01N 27/48 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01); G01N 2800/00 (2013.01);
Abstract

This invention is based, in part, on our discovery of an essentially one-step, label-free system comprising a sensing unit having a redox current reporter and a nucleic acid sequence complementary to that of a target nucleic acid of interest or sufficiently complementary to that of the target nucleic acid or a sequence therein to specifically bind the target nucleic acid. The sensing unit is bound to an electroconductive substrate (e.g., a carbon- or metal-containing microelectrode (e.g., a gold microelectrode)), and the system includes a signal amplification mechanism that does not rely upon a redox enzyme and thereby overcomes a fundamental limitation of microelectrode DNA sensors that fail to generate detectable current in the presence of only small amounts of a target nucleic acid.


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