The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Jun. 21, 2016
Applicants:

University of Washington Through Its Center of Commercialization, Seattle, WA (US);

Perkinelmer Health Sciences, Inc., Waltham, MA (US);

Inventors:

Alexander Cherkassky, Wayland, MA (US);

Jason Coumoyer, Jamaica Plain, MA (US);

Michael Gelb, Seattle, WA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/34 (2006.01); C07C 233/18 (2006.01); C07H 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/34 (2013.01); C07C 233/18 (2013.01); C07H 15/10 (2013.01); G01N 2333/924 (2013.01);
Abstract

Methods are provided for detecting enzymatic activity of various lysosomal storage enzymes using substrates that include: a sugar moiety; a linker moiety allowing the conjugation of sugar moiety with the remaining structure of the substrate; and two or more fatty acid chains or derivatives thereof at least one of which is sufficiently structured to provide improved solubility in aqueous or organic solvent systems. Also provided are internal standards, and inhibitors for use in detecting or reducing enzymatic activity using the inventive substrates.


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