The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 26, 2021
Filed:
May. 14, 2015
Universidad DE Los Andes, Bogotá, CO;
Corporación Corpogen, Bogotá, CO;
Alvaro Mongui, Bogotá, CO;
Patricia Del Portillo Obando, Bogotá, CO;
Silvia Restrepo Restrepo, Bogotá, CO;
Armando Junca Howard, Cundinamarca, CO;
UNIVERSIDAD DE LOS ANDES, Bogota, CO;
CORPORACION CORPOGEN, Bogota, CO;
Abstract
A transposon (TnC_T7) was developed to partially supply the transcriptional machinery during functional analysis of genomic/metagenomic libraries. This transposon was conceived and constructed to have the ability to integrate randomly into any episomal DNA, allowing the inducible expression of the adjacent DNA regions in both directions. In general, this genetic tool included a kanamycin resistance gene, two bidirectional T7 promoters and the T7RNA polymerase-coding gene, the latter under the regulation of the inducible arabinose promoter (P). The experimental validation confirmed the TnC_T7 potential to be used in functional genomic/metagenomic studies, in order to partially overcome the limitations of the bacterial hosts, which prevent them to recognize most of foreign genes from DNA libraries.