Target nucleic acid concentration and recovery method using antibody

Abstract

An object of the present invention is to provide an efficient nucleic acid recovery method capable of concentrating a nucleic acid from a sample containing a nucleic acid such that the nucleic acid is usable in gene amplification reaction. To solve the problem, the present inventors have found that by targeting a protein to which a nucleic acid is bound (hereinafter also referred to as a nucleoprotein or a protein-nucleic acid complex) rather than targeting the nucleic acid itself, or specifically, by causing a specific antibody against the protein to act, the nucleic acid can be captured simultaneously with the protein, and thereby completing the present invention. Furthermore, the present inventors have found that if animal cells, bacteria, viruses, etc. have a nucleic acid bound to protein present in a cell (in an envelope in the case of viruses), the bound nucleic acid can be captured and recovered by using a specific antibody against the nucleoprotein without dissociating the nucleic acid from the protein after treating the cell with a surfactant.