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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11155794 B1

PDFFull Text
Date of Patent:
Oct. 26, 2021

Filed:

Aug. 22, 2017

Method of purifying a heterologous protein from an egg white

Applicant:

Alexion Pharmaceuticals, Inc., Boston, MA (US);

Inventors:

Liang Chen, San Diego, CA (US);

Markley C. Leavitt, Lexington, MA (US);

Michael Titus, Lexington, MA (US);

Stephen Palmieri, Foxboro, MA (US);

Assignee:

ALEXION PHARMACEUTICALS, INC., Boston, MA (US);

Attorney:

Medler Ferro Woodhouse & Mills

Primary Examiner:

Erin M Bowers

Int. Cl.
CPC ...
C12N 9/20 (2006.01); C07K 1/20 (2006.01); C07K 1/34 (2006.01); C07K 1/36 (2006.01); B01D 15/18 (2006.01); B01D 15/24 (2006.01); B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01D 61/14 (2006.01); C07K 1/18 (2006.01);
U.S. Cl.
CPC ...
C12N 9/20 (2013.01); B01D 15/1871 (2013.01); B01D 15/24 (2013.01); B01D 15/327 (2013.01); B01D 15/362 (2013.01); B01D 61/145 (2013.01); C07K 1/18 (2013.01); C07K 1/20 (2013.01); C07K 1/34 (2013.01); C07K 1/36 (2013.01); B01D 2311/18 (2013.01); B01D 2311/2626 (2013.01); B01D 2315/16 (2013.01);
Abstract

The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and collecting a second eluate comprising the heterologous protein.

Find Patent Forward Citations

Quick Actions:

Chen, Liang. (2021). Method of purifying a heterologous protein from an egg white (U.S. Patent No. 11155794). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11155794

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