The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Mar. 13, 2014
Applicant:

Glaxosmithkline Biologicals SA, Rixensart, BE;

Inventors:

Francesco Berlanda Scorza, Holly Springs, NC (US);

Yingxia Wen, Cambridge, MA (US);

Andrew Geall, Cambridge, MA (US);

Frederick Porter, Holly Springs, NC (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07H 21/02 (2006.01); B01D 61/14 (2006.01); B01D 15/38 (2006.01); C12N 15/10 (2006.01); B01D 15/36 (2006.01); C07H 1/06 (2006.01);
U.S. Cl.
CPC ...
C07H 21/02 (2013.01); B01D 15/36 (2013.01); B01D 15/38 (2013.01); B01D 15/3847 (2013.01); B01D 61/14 (2013.01); C07H 1/06 (2013.01); C12N 15/101 (2013.01); C12N 15/1017 (2013.01); B01D 2015/3838 (2013.01);
Abstract

Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.


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