The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Apr. 19, 2017
Applicant:

Integrated Research Associates, Llc, San Rafael, CA (US);

Inventors:

Mary Jane Cardosa, Penang, MY;

Katharine Bossart, San Francisco, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/29 (2006.01); C12N 7/00 (2006.01); A61K 39/12 (2006.01); C12N 9/64 (2006.01); A61P 31/14 (2006.01); C12N 15/62 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
A61K 39/29 (2013.01); A61K 39/12 (2013.01); A61P 31/14 (2018.01); C12N 7/00 (2013.01); C12N 9/6454 (2013.01); C12N 15/62 (2013.01); A61K 2039/55505 (2013.01); C12N 2710/14143 (2013.01); C12N 2770/24123 (2013.01); C12N 2770/24134 (2013.01); C12N 2770/24151 (2013.01); C12N 2800/22 (2013.01); C12N 2830/20 (2013.01); C12N 2830/50 (2013.01); C12N 2840/203 (2013.01); Y02A 50/30 (2018.01);
Abstract

The present invention relates to the production of structural proteins and virus-like particles (VLPs) of flaviviruses and hepatitis C. Production is through use of a single expression cassette comprising a viral structural gene, a furin encoding gene and a bicistronic expression element such as an internal ribosome entry site (IRES) between the viral and furin genes. Both the viral gene and furin gene can include a partial capsid encoding sequence acting as a signal peptide to co-locate the viral protein and furin and to act as a membrane anchor for the viral protein and furin. A separate expression cassette comprising a non-structural viral gene can be combined with the initial cassette. The structural proteins and VLPs can be used in vaccines and in the treatment of viral infections.


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