The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2021

Filed:

Jul. 22, 2019
Applicant:

Wayne State University, Detroit, MI (US);

Inventors:

Jianjun Wang, Troy, MI (US);

Qianqian Li, Troy, MI (US);

Assignee:

Wayne State University, Detroit, MI (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/00 (2006.01); C12N 15/88 (2006.01); G01N 33/50 (2006.01); A61K 47/54 (2017.01); A61K 47/58 (2017.01); C12P 21/00 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
A61K 38/00 (2013.01); A61K 47/543 (2017.08); A61K 47/58 (2017.08); C12N 15/88 (2013.01); C12P 21/00 (2013.01); G01N 33/5023 (2013.01); G01N 33/58 (2013.01); G01N 33/68 (2013.01); G01N 2458/15 (2013.01);
Abstract

A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.


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