The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 19, 2021

Filed:

Apr. 23, 2018
Applicant:

Helmholtz Zentrum München—deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh), Neuherberg, DE;

Inventor:

Ralf Kühn, Berlin, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); A01K 67/027 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C12N 9/22 (2006.01); C12N 5/075 (2010.01); C12N 9/16 (2006.01); C07K 14/195 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); A01K 67/0275 (2013.01); C07K 14/195 (2013.01); C12N 5/0609 (2013.01); C12N 9/16 (2013.01); C12N 9/22 (2013.01); C12N 15/62 (2013.01); C12N 15/8509 (2013.01); C12Y 301/21004 (2013.01); A01K 2207/05 (2013.01); A01K 2217/07 (2013.01); A01K 2217/075 (2013.01); A01K 2227/105 (2013.01); A01K 2267/03 (2013.01); C07K 2319/80 (2013.01); C12N 2800/80 (2013.01); C12N 2999/007 (2013.01);
Abstract

The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease. Also, the present invention relates to a vector comprising the nucleic acid molecule and a protein encoded by said nucleic acid molecule. Further, the invention relates to a method of modifying the genome of a eukaryotic cell and a method of producing a non-human vertebrate or mammal.


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