The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 19, 2021

Filed:

Dec. 15, 2016
Applicant:

Dsm Ip Assets B.v., Heerlen, NL;

Inventors:
Assignee:

DSM IP ASSETS B.V., Heerlen, NL;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 15/102 (2013.01); C12N 15/111 (2013.01); C12N 15/902 (2013.01); C12N 2310/20 (2017.05); C12Q 2521/301 (2013.01);
Abstract

The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.


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