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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11149246 B1

PDFFull Text
Date of Patent:
Oct. 19, 2021

Filed:

Mar. 07, 2019

Methods for cell lysis and preparation of high molecular weight dna from modified cells

Applicant:

Shoreline Biome, Llc, Farmington, CT (US);

Inventors:

Mark Driscoll, Wallingford, CT (US);

Thomas Jarvie, Branford, CT (US);

Assignee:

Shoreline Biome, LLC, Farmington, CT (US);

Attorneys:

Wiggin and Dana LLP

Anthony D. Sabatelli

Madeleine E. Tavcar

Primary Examiner:

Frank W Lu

Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 1/06 (2006.01); C12Q 1/686 (2018.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 1/06 (2013.01); C12N 15/11 (2013.01); C12Q 1/686 (2013.01); C12N 2310/20 (2017.05);
Abstract

Disclosed are methods for lysis of cells that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allow for simplified removal of detergent after lysis, and importantly, limits damage to DNA, such as shearing, that typically results from separate application of conventional lysis methods, yielding improved quality and quantities of genomic DNA (gDNA).

Find Patent Forward Citations

Driscoll, Mark. (2021). Methods for cell lysis and preparation of high molecular weight dna from modified cells (U.S. Patent No. 11149246). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11149246

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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