The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 2021
Filed:
Mar. 07, 2019
Applicant:
Shoreline Biome, Llc, Farmington, CT (US);
Inventors:
Mark Driscoll, Wallingford, CT (US);
Thomas Jarvie, Branford, CT (US);
Assignee:
Shoreline Biome, LLC, Farmington, CT (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 1/06 (2006.01); C12Q 1/686 (2018.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 1/06 (2013.01); C12N 15/11 (2013.01); C12Q 1/686 (2013.01); C12N 2310/20 (2017.05);
Abstract
Disclosed are methods for lysis of cells that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allow for simplified removal of detergent after lysis, and importantly, limits damage to DNA, such as shearing, that typically results from separate application of conventional lysis methods, yielding improved quality and quantities of genomic DNA (gDNA).