The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 12, 2021

Filed:

Sep. 20, 2018
Applicant:

Pacific Biosciences of California, Inc., Menlo Park, CA (US);

Inventors:

Stephen Turner, Eugene, OR (US);

Jonas Korlach, Camas, WA (US);

Satwik Kamtekar, Mountain View, CA (US);

Jeremiah Hanes, Woodside, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); B82Y 10/00 (2011.01); B82Y 15/00 (2011.01); C12Q 1/6874 (2018.01); G01N 27/414 (2006.01); G11C 19/28 (2006.01); H01L 29/06 (2006.01); H01L 29/16 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B82Y 10/00 (2013.01); B82Y 15/00 (2013.01); C12Q 1/6874 (2013.01); G01N 27/4145 (2013.01); G01N 27/4146 (2013.01); G01N 27/4148 (2013.01); G11C 19/28 (2013.01); H01L 29/0669 (2013.01); H01L 29/1606 (2013.01); H01L 29/0673 (2013.01);
Abstract

Real time electronic sequencing devices, chips, and systems are described. Arrays of nanoFET devices are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound to the nanoFET. The nanoFET devices typically have a source, a drain and a gate comprising a nanowire. A single polymerase enzyme complex comprising a polymerase enzyme complexed with the template nucleic acid is bound to the gate. The polymerase is bound to the gate non-covalently through a polymeric binding agent that has two strands, each strand interacting with the nanowire such that the polymerase is in a central location between the strands with the polymeric binding agent extending away from the polymerase complex along the nanowire in both directions.


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