The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 12, 2021

Filed:

Apr. 28, 2015
Applicants:

Juewen Liu, Kitchener, CA;

Po-jung Jimmy Huang, Taipei, TW;

Inventors:

Juewen Liu, Kitchener, CA;

Po-Jung Jimmy Huang, Taipei, TW;

Assignee:

Other;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12N 15/11 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C07H 21/04 (2013.01); C12N 15/10 (2013.01); C12N 15/111 (2013.01); C12Q 1/68 (2013.01); C12N 2310/127 (2013.01); C12N 2310/315 (2013.01); C12N 2310/3519 (2013.01); C12N 2310/531 (2013.01); C12N 2320/10 (2013.01);
Abstract

A novel substrate-bound DNAzyme complex is provided comprising a DNAzyme bound to a nucleic acid-based substrate. The DNAzyme comprises a pair of binding arms which hybridize to binding regions on the substrate, and a catalytic domain between the binding arms. The nucleic acid-based substrate comprises a phosphorothioate-modified ribonucleotide cleavage site between the binding regions of the substrate. The catalytic domain of the DNAzyme catalyzes heavy metal-dependent cleavage of the substrate cleavage site. The DNAzyme complex is useful in a method of heavy metal sensing. A novel cadmium-selective DNAzyme is also described for cadmium sensing.


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