The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 05, 2021

Filed:

Feb. 12, 2019
Applicant:

Shimadzu Corporation, Kyoto, JP;

Inventors:

Yuko Fukuyama, Kyoto, JP;

Hiroto Tamura, Kani, JP;

Teruyo Kato, Aisai, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); H01J 49/00 (2006.01); C12Q 1/04 (2006.01); G01N 33/68 (2006.01); G16B 50/00 (2019.01); H01J 49/16 (2006.01); G16B 40/10 (2019.01);
U.S. Cl.
CPC ...
G01N 33/569 (2013.01); C12Q 1/04 (2013.01); G01N 33/6851 (2013.01); G16B 50/00 (2019.02); H01J 49/0027 (2013.01); H01J 49/164 (2013.01); G01N 2560/00 (2013.01); G16B 40/10 (2019.02);
Abstract

In a method for analyzing a microorganism using a matrix assisted laser desorption/ionization mass spectrometer, a matrix-and-additive mixture solution prepared by mixing one or both of an alkylphosphonic acid and a surfactant with a matrix substance is used for matrix assisted laser desorption/ionization. Either an alkylphosphonic acid or a surfactant, or both of them are used as matrix additives and are mixed with the matrix substance beforehand to prepare a matrix-and-additive mixture solution. After a solution which contains a microorganism to be analyzed has been dropped onto a sample plate, the matrix-and-additive mixture solution is dropped onto that solution and dried to form a mixed crystal which contains both the constituents of the microorganism and the matrix substance. This crystal is used as a sample for MALDI-MS analysis. The sensitivity of analysis is thereby improved, without increasing the amount of time and labor required for sample preparation.


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