The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2021
Filed:
Oct. 07, 2016
Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);
Jacob Messner, Mesa, AZ (US);
Clifford Anderson, Tempe, AZ (US);
Honor Glenn, Mesa, AZ (US);
Kristen Lee, Mesa, AZ (US);
Mark Richards, Chandler, AZ (US);
Laimonas Kelbauskas, Gilbert, AZ (US);
Kimberly Bussey, Phoenix, AZ (US);
Deirdre Meldrum, Phoenix, AZ (US);
Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);
Abstract
Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.