The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 05, 2021

Filed:

Jan. 25, 2017
Applicant:

Pioneer Hi-bred International, Inc., Johnston, IA (US);

Inventors:

Andrew Mark Cigan, Madison, WI (US);

Mark Jacob Gadlage, Dyersburg, TN (US);

Huirong Gao, Johnston, IA (US);

Robert B Meeley, Des Moines, IA (US);

Joshua K Young, Johnston, IA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); A01H 5/10 (2018.01); C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8245 (2013.01); A01H 5/10 (2013.01); C12N 9/1051 (2013.01); C12N 15/8213 (2013.01); C12N 2310/20 (2017.05);
Abstract

The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.


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