The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2021
Filed:
Jun. 24, 2016
Applicant:
Whitehead Institute for Biomedical Research, Cambridge, MA (US);
Inventors:
Assignee:
Whitehead Institute for Biomedical Research, Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01); C12N 5/00 (2006.01); C12N 5/0793 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0622 (2013.01); C12N 5/0025 (2013.01); C12N 5/0618 (2013.01); C12N 5/0619 (2013.01); G01N 33/5058 (2013.01); C12N 2500/05 (2013.01); C12N 2500/24 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12N 2500/60 (2013.01); C12N 2502/081 (2013.01); C12N 2502/086 (2013.01); C12N 2506/02 (2013.01); C12N 2506/03 (2013.01); C12N 2506/45 (2013.01);
Abstract
Described herein are cell culture media useful for the differentiation of human pluripotent stem cells into microglia. The methods described herein relate to in vitro generation of expandable, bankable, microglial cells by directed differentiation from human pluripotent stem cells (induced or embryonic). Using only defined cell culture media, differentiation of pluripotent stem cells is directed down a mesodermal path, in a rapid and scalable fashion, to generate cells adopting signatures of their in vivo counterparts, including gene expression, protein marker expression and functionality.