The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2021
Filed:
Dec. 18, 2018
Jiangnan University, Wuxi, CN;
Long Liu, Wuxi, CN;
Xueqin Lv, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Jian Chen, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The invention provides a recombinant, construction method and use thereof, wherein the cell's own FMMs are used as a space scaffold, and a multi-enzyme complex is constructed from specific marker proteins FloA and FloT, such that an artificial substrate channel is formed, and the cell metabolic burden is effectively reduced. The recombinantof the invention can efficiently synthesize GlcNAc without affecting cell life activity, and can also limit the toxic intermediate metabolite GlcN-6-P near the plasma membrane to reduce or eliminate its inhibition on cell activity. In the process of shaking flask fermentation using complex medium, the yield of acetyl glucosamine of the control strain BSG-C was only 0.45 g·L, while that of BSG-AT, BSG-ATP, BSG-ATPB increased to 5.29 g·L, 6.22 g·Land 8.48 g·Lrespectively. The construction method of recombinantis simple, easy to use and has a good application prospect.