The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 05, 2021

Filed:

Jun. 14, 2019
Applicant:

Industry-university Cooperation Foundation Hanyang University Erica Campus, Ansan-si, KR;

Inventors:

Dong Wook Kim, Seoul, KR;

Eun Jin Kim, Seoul, KR;

Hyun Jin Yu, Incheon, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/00 (2006.01); A61K 39/02 (2006.01); A61K 39/106 (2006.01); C07K 14/28 (2006.01); C12N 15/74 (2006.01); C12N 7/00 (2006.01); C12N 15/65 (2006.01); C12P 21/02 (2006.01); C12N 1/20 (2006.01); C12R 1/63 (2006.01);
U.S. Cl.
CPC ...
C07K 14/28 (2013.01); C12N 1/205 (2021.05); C12N 7/00 (2013.01); C12N 15/65 (2013.01); C12N 15/74 (2013.01); C12P 21/02 (2013.01); C12N 2795/00042 (2013.01); C12R 2001/63 (2021.05);
Abstract

The present invention relates to a plasmid-based CTX phage replication system andstrain that can be infected by CTX phage and can be used for cholera toxin production. More particularly, the present invention provides avariant strain, which expresses a toxT protein in which tyrosine at position 139 is substituted by phenylalanine through the point mutation of a toxT gene using a plasmid-based CTX phage replication system, and is used as a receptor strain which can improve CTX phage infection efficiency and allows a plurality of CTX prophages to simultaneously infect the strain and to be inserted into the chromosome thereof, which the consequent provision of the effect of increasing the production yield of a cholera toxin.


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