The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2021
Filed:
Oct. 13, 2016
University of Virginia Patent Foundation, Charlottesville, VA (US);
James P. Landers, Charlottesville, VA (US);
Jacquelyn A. DuVall, Raleigh, NC (US);
Delphine Le Roux, Barboursville, VA (US);
Brian Root, Charlottesville, VA (US);
Daniel Mills, Charlottesville, VA (US);
Daniel A. Nelson, Charlottesville, VA (US);
An-Chi Tsuei, Charlottesville, VA (US);
Brandon L. Thompson, Charlottesville, VA (US);
Jingyi Li, Charlottesville, VA (US);
Christopher Birch, Charlottesville, VA (US);
University of Virginia Patent Foundation, Charlottesville, VA (US);
Abstract
A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.