The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 28, 2021

Filed:

Jul. 04, 2014
Applicant:

Novozymes A/s, Bagsvaerd, DK;

Inventors:

Michael Dolberg Rasmussen, Bagsvaerd, DK;

Gitte Bak Poulsen, Bagsvaerd, DK;

Assignee:

Novozymes, A/S, Bagsvaerd, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 15/10 (2006.01); C12N 15/75 (2006.01);
U.S. Cl.
CPC ...
C12N 15/902 (2013.01); C12N 15/1082 (2013.01); C12N 15/75 (2013.01);
Abstract

The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.


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