The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 28, 2021
Filed:
Dec. 21, 2017
Applicant:
The University of Hong Kong, Hong Kong, HK;
Inventors:
Hei Ming Lai, Hong Kong, HK;
Wutian Wu, Hong Kong, HK;
Assignee:
THE UNIVERSITY OF HONG KONG, Hong Kong, HK;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/34 (2006.01); C11D 7/32 (2006.01); C11D 7/10 (2006.01); C11D 7/26 (2006.01); C11D 7/34 (2006.01);
U.S. Cl.
CPC ...
C11D 7/3272 (2013.01); C11D 7/10 (2013.01); C11D 7/263 (2013.01); C11D 7/3209 (2013.01); C11D 7/3263 (2013.01); C11D 7/34 (2013.01); G01N 1/34 (2013.01);
Abstract
A composition and a method for clearing tissue for subsequent three-dimensional analysis. The clearing composition comprises: (1) a homogenizing agent such as N-methylglucamine, urea, or ethylenediamine; (2) a cytoplasmic, water-soluble refractive index adjusting agent such as iohexol, sodium thiosulfate, or polyethylene glycol; and (3) a membrane, lipid-soluble RI adjusting agent such as 2,2'-thiodiethanol (TDE) or propylene glycol. The tissue clearing composition is particularly suitable for use with long-term fixed human tissues.